Figure 5

Berberine induced autophagy in J774A.1 cells was mediated by AMPK/mTOR pathway. (A) Representative blots of mTOR in BBR treated J774A.1 cells which were incubated with mTOR inhibitor rapamycin (Rap) or mTOR agonist leucine (LEU). (B) Bars graphs showed the quantification of indicated proteins. (C) Representative blots of LC3 and SQSTM1/p62 in ox-LDL stimulated J774A.1 cells treated with berberine in the presence or absence of 10 μM CC. (D) Bars graphs showed the quantification of indicated proteins. (E) Representative blots of P-AMPK/AMPK and p-mTOR/mTOR in J774A.1 cells treated with berberine in the presence or absence of 10 μM CC. (F) Bar graphs showed the quantification of indicated proteins. Experiments were repeated at least three times. **P < 0.01 V.S. control group, *P < 0.05 V.S. control group; ##P < 0.01 V.S. ox-LDL group, #P < 0.05 V.S. ox-LDL group; $$P < 0.01 V.S.BBR group, $P < 0.05 V.S.BBR group; aa P < 0.01 V.S. BBR group, a P < 0.05 V.S. BBR group; bb P <0.01 V.S.L-L group.