Figure 5

HIF2α mediated the function of hypoxia in promoting cardiac differentiation. (A) The percentages of beating EBs derived from scramble shRNA or shHIF2α ESCs in the presence of absence of 100 μm CoCl2. Data represent the mean ± s.d. of three biological replicates. *p < 0.05, **p < 0.01 vs Scramble-NT. NT: No Treatment. (B) qRT-PCR analysis of Gata4 and Nkx2.5 mRNA levels in scramble shRNA or shHIF2α EBs treated with or without CoCl2. Data represent the mean ± s.d. of three biological replicates. *p < 0.05, **p < 0.01 vs Scramble-NT. NT: No Treatment. (C) Western blot analysis of the protein levels of HIF1α or HIF2α in 46C mouse ESCs treated with or without 100 μM CoCl2 for 10 h. (D) qRT-PCR analysis of HIF1α or HIF2α mRNA levels in HIF1α and HIF2α double knockdown ESCs. Data represent the mean ± s.d. of three biological replicates. **p < 0.01 vs Scramble. (E) The beating frequency of EBs was analyzed among the indicated groups at day 15. Data represent the mean ± s.d. of three biological replicates. **p < 0.01 vs Scramble-NT.