Figure 2

AKT activation in ischemic hearts. A: Illustrative representation of all experimental groups. “IR”, Ischemia-reperfusion. “Pre”, SC79 administered before ischemia. “Post”, SC79 administered at reperfusion. “Both”, SC79 administered before ischemia and at reperfusion. “Low” indicates 100nM SC79 and “High” indicates 300nM SC79. B: AKT phosphorylation in normoxic-perfused (N) or ischemic hearts (IR) treated with DMSO or SC79. C: Total AKT expression; D: Correlation between AKT phosphorylation and GSK-3β phosphorylation. E: Correlation between AKT phosphorylation and Ribosomal-S6 phosphorylation. The Pearson correlation test was used to analyze the correlations shown in panels D and E. Number of animals analyzed per group is displayed within the bars. Western blot bands displayed in panels D and E are disposed in the same group order as in panels B and C. Data are shown as mean ± SE. #, p < 0.05 vs. IR-Control; &, p < 0.05 vs. all other groups.