HOTTIP knockdown inhibits HOXA13 expression. (A)
(B) MIA PaCa-2 and SW1990 cells were transduced with control shRNA or shRNA against HOTTIP as indicated. Knockdown of HOTTIP abrogated the expression of HOXA genes in the PDAC cell lines, with the strongest inhibition observed for HOXA13. (C) The effect of HOTTIP knockdown on HOXA13 protein levels was evaluated by western blotting. (D) The effect of HOTTIP on HOXA13 expression was measured by immunofluorescence. (E)
HOXA13 expression levels were examined in the above-mentioned paired PDAC tissues and their adjacent non-neoplastic tissues by PCR. (F) Correlation scatterplot (Spearman test) of HOTTIP and HOXA13 expression in PDAC (middle panel; B2) compared with para-tumor (right panel; B3) areas of the above tested tissues.