Figure 7

TS down-regulates RANKL-induced expression of NFTAc1 and c-Fos in RAW 264.7 cells. RAW 264.7 cells were preincubated with indicated concentrations of TS for 2 h, and then treated with 50 ng/mL RANKL for 24 h. Total protein was extracted and subjected to Western blot analysis using antibodies against NFATc1 and c-Fos (A). Relative amounts of each protein were determined by densitometric analysis (B). One of three experiments with similar results is shown. Data represent the mean ± SD of three independent experiments. ^### P < 0.001 significantly different from basal. *P < 0.05, **P < 0.01 and ***P < 0.001 significantly different from RANKL only group.