Figure 1

TS inhibits RANKL-induced osteoclast differentiation. (A) RAW 264.7 cells were cultured in the presence of RANKL with different concentrations of TS (0.1, 0.5 and 2.5 μg/mL). Six days post-culture, cells were fixed with 4% paraformadehyde followed by TRAP staining. Representative images of TRAP staining of OCLs from one of the three experiments are shown. (B) Quantitative analysis show the mean number of TRAP-positive OCLs, mean OCLs nuclei numbers, and mean OCLs sizes. (C) BMMs were cultured in the presence of RANKL with different concentrations of TS (0.1, 0.5 and 2.5 μg/mL). Six days post-culture, cells were fixed with 4% paraformadehyde followed by TRAP staining. All bar graphs represent mean ± SD of three independent experiments. ^### P < 0.001 significantly different from Control. *P < 0.05, **P < 0.01 and ***P < 0.001 significantly different from RANKL only group.