Figure 3

miR-130a sensitized the response of glioma cells to temozolomide (TMZ). (A) miR-130a level in 5 glioblastoma multiforme(GBM) cell lines. (B) SHG44 was transfected with miR-130a mimics (SHG44-M) and scrambled control miRNAs (SHG44-SC). (C) SHG44-M were more sensitive to TMZ than SHG44-SC (p = 0.027). Overexpression of miR-130a increased PARP cleavage in SHG44 induced by TMZ. (D) U251 was transfected with inhibitors for miR-130a (U251-I) and the scrambled control (U251–SC). (E) U251-I were more resistant to TMZ than U251–SC (p = 0.039). Knockdown of miR-130a decreased PARP cleavage in U251 induced by TMZ.