Figure 2

Effect of ex vivo transformation of LLC cells with the TK and TKmGM constructs combined with administration of GCV on A) tumor growth rate, and B) animal lifespan after transplantation of the transfected cells into C57Bl/6 mice. The data represent mean values for treatment groups of ten animals and control groups of six animals. We studied 6 groups of mice: two control groups (K/GCV and K/PBS) inoculated with non-transfected cells; two experimental groups (TK/GCV and TK/PBS) inoculated with LLC cells transfected with TK; and two experimental groups (TKmGM/GCV and TKmGM/PBS) inoculated with LLC cells transfected with TKmGM using LFA. The animals of groups К/GCV, ТK/GCV and TKmGM/GCV received intraperitoneal injections of ganciclovir in a dose of 75 mg/kg twice a day for 10 days. The animals of groups К/PBS, ТK/PBS and TKmGM/PBS received phosphate buffered saline (PBS) as a placebo instead of GCV. Starting from day 6 after transplantation, we measured the volume of developed tumors. The euthanasia criterion was the tumor volume that exceeded 2000 mm³. A) Tumor volume (in mm³, Y-axis) versus time since cell transplantation (X-axis). Mean ± SEM values are shown. B) Survival period of mice after transplantation of the transfected and non-transfected LLC cells.