Figure 6
From: PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL
PEDF and 44mer-induced TG degradation depend on the ATGL lipolysis activity . A: western blots for ATGL of untreated control cell samples, samples treated with ATGL overexpression, and samples treated with ATGL siRNA compared with β-actin are shown. B: densitometry of the western blots shown in (A) normalized to β-actin. n = 3, values are means ± SD. *P < 0.05 compared with control samples. **P < 0.05 compared with control samples and ATGL group. C: oil red O staining of untreated control cardiomyocytes, cardiomyocytes treated with siATGL + rPEDF, cardiomyocytes treated with siATGL + 44mer, cardiomyocytes treated with ATGL + rPEDF and cardiomyocytes treated with ATGL + 44mer. Scale bar, 20 μm. D: quantification of TG content of cardiomyocytes samples shown in (A), *P < 0.05 compared with control group. n = 3, Values are means ± SD. E: quantification of TG content in the presence of ATGL inhibitor atglistatin. n = 4, *P < 0.05 compared with control group; #P < 0.05 compared with hypoxia group. Values are means ± SD.