Expression of PEDF and ATGL in the rat heart. A and C: Expression of PEDF and ATGL protein 2, 4 and 6 weeks after infarction. Upper panels show representative results of PEDF and ATGL immunostaining. Lower panels show the quantitative data of PEDF and ATGL. β-actin was used as a loading control. The bands were quantified using the Image J software, and the data were transformed and normalized to β-actin. n = 3 per group. W indicates weeks. Values are means ± SD. *P < 0.05 compared with normal group. B and D: PEDF and ATGL mRNA expression in infarcted area 2, 4 and 6 weeks after infarction by qPCR analysis. NADPH was used as an internal control. n = 4, Values are means ± SD. *P < 0.05 compared with normal group.