Figure 11

ASC-CM suppresses activation of pro-apoptotic pathways in HPAEC. HPAEC were treated with vehicle control, NHDF-CM, and hASC-CM, and then stimulated with indicated concentration of TNFα (4 h). Cell lysates were analyzed with antibodies to cleaved caspase 3, Bad, phospho-Bad, Bim, and Bcl2. β-actin staining was used as loading control.