Figure 10

ASC-CM suppresses ICAM-1 and E-selectin expression by HPAEC. HPAEC were treated with vehicle control (NoPr, black bars), NHDF-CM (DF-CM, grey bars), and hASC-CM (ASC-CM, white bars), and then stimulated with vehicle or 1 ng/ml TNFα (4 h). Harvested cells were analyzed for the surface expression of ICAM-1 and E-selectin. Experiments with independent treatment/stimulation were repeated in triplicate. T-test was used to assess the differences between analyzed groups. Exposure to hASC-CM lowers the percentage of ICAM-1-expressing quiescent HPAEC and reduces the level of E-selectin expression in TNFα-stimulated HPAEC.