Figure 5

Knockdown of STC1 impairs epithelial-mesenchymal transition (EMT) of Caki-1 and A498 cells. (A) (B) Forty-eight hours after transfection, a series of EMT-related markers, including the mesenchymal markers N-cadherin, Vimentin, α-SMA, ZEB1, and the epithelial marker E-cadherin were determined by Western blotting and immunofluorescence staining (magnification, ×600) Red, CF555; blue, DAPI. (C) Western blotting results for the protein expression alteration of several EMT-related markers. **,P < 0.01; ***,P < 0.001.