Figure 1

Schematic drawing showed a series of studies using caspase-3 siRNA. The caspase-3 siRNA was first used to protect porcine renal tubular epithelia cells against hydrogen peroxide-induced injury. The renoprotection of naked caspase-3 siRNA with the same sequences was further validated in a porcine ex vivo isolated reperfusion model, then shown that the siRNA was effective for cold preservation, but not in auto-transplanted kidneys without systematic siRNA treatment. Finally, the modified siRNA of caspase-3 via locked nucleic acid stabilized the siRNA in serum, and significantly protected auto-transplanted kidneys.