Figure 7

Immunohistochemical (IHC) ( 200×) staining of alpha- smooth muscle actin for quantification of small vessel (≤25.0 μm) in ischemic quadriceps at day 14 after CLI induction (n =  8 ). A to E) Indicating IHC stain of number of small vessels (red arrows) in five groups. F) * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. Scale bars in right lower corner represent 50 μm. G to I) Demonstrating the H.E. staining for the lung parenchyma in SC + Lc-MPs (G), CLI + Hs-MPs (H), and CLI + Lc-MPs. No tumorigenesis was found in these three groups of animals. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC = sham control; CLI = critical limb ischemia; Lc-MPs = lung-cancer patient-derived microparticles; Hs-MPs = Healthy subject-derived microparticles.