Figure 6

Immunofluorescence (IF) microscope (400×) findings of vascular endothelial growth factor (VEGF) ⁺ and hepatocyte growth factor (HGF) ⁺ stained cells in ischemic quadriceps by day 14 following CLI procedure (n =  8) . A to E) Showing the IF stain of VEGF⁺ cells (white arrows) in five groups. F) * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. G to K) Illustrating the IF stain of HGF⁺ cells (white arrows) in five groups. L) * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. Scale bars in right lower corner represent 20 μm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC = sham control; CLI = critical limb ischemia; Lc-MPs = lung-cancer patient-derived microparticles; Hs-MPs = Healthy subject-derived microparticles.