Figure 2
From: Postconditioning in major vascular surgery: prevention of renal failure
Anti-myoglobin immunohistochemistry (Gill’ s hematoxylin as background staining, 200x and 600x magnification). A: Kidney section of an IR (ischemia-reperfusion) animal (4 h after revascularization) shows myoglobin casts in the lumen of the tubuli, anti-myoglobin-positive vacuoli within the proximal tubular cells. All other stain was considered non-specific binding and negative for the presence of myoglobin. B: Slide of a PostC (postconditioned) animal 4 h after revascularization. Less anti-myoglobin positivity can be detected, C: IR animal 24 h after revascularization, myoglobin epitopes were absent.