Table 2 Standardized antibody panel
Leukocyte population | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Fluorochrome (directly conjugated to monoclonal antibody) | ||||||||||||
FITC | PE | PE-Cy5 | PerCP-Cy5.5 | PE-TR | PE-Cy7 | APC | APC-Cy7 | V500 | Pacific blue | AF 700 | QDot 655 | |
Surface antigen detected with monoclonal antibody clone used (italicized) | ||||||||||||
Dendritic cells# | BDCA-2 AC144 | BDCA-1 AD5-8E7 | CD123 6H6 | CCR2 TG5 | CD45 HI30 | CD103 B-Ly7 | BDCA-3 AD5-14H12 | CX3CR1 2A9-1 | HLA-DR L243 | CD11c 3.9 | CD11b CBRM1/5 | CD3 7D6 & CD19 SJ25-C1 |
Mø & monocytes | CD14 HCD14 | CCR6 R6H1 | CD16 3G8 | CCR2 TG5 | CD45 HI30 | TLR2 T2.5 | CD206 15-2 | CX3CR1 2A9-1 | HLA-DR L243 | CD11c 3.9 | CD11b CBRM1/5 | |
Mø & monocytes | CD14 HCD14 | Axl 108737 | CD16 3G8 | DC-Sign 9E9A8 | CD45 HI30 | TLR4 HTA125 | Mertk 125518 | HLA-DR L243 | CD11c 3.9 | CD11b CBRM1/5 | ||
Basophils*# | CD33 HIM3-4 | CD9C3-3A2 | CD13 TuK1 | CD203c NP4D6 | CD45 HI30 | CD63 H5C6 | CD22 HIB22 | CD34 581 | HLA-DR L243 | CD69 FN50 | CD11b CBRM1/5 | CD19 SJ25-C1 |
Eosinophils | CD49d 9 F10 | CDw125 A14 | CD16 3G8 | CD69 FN50 | CD45 HI30 | CCR3 5E8 | CD34 581 | CD11b CBRM1/5 | ||||
Neutrophils | CD177 MEM-166 | CD16b CLB-gran 11.5 | CXCR1 8 F1 | CD66b G10F5 | CD45 HI30 | TLR4 HTA125 | CXCR2 5E8 | CD10 HI10a | TLR2 T2.5 | CD11b CBRM1/5 | ||
Endothelial cells*# | CD33 HIM3-4 | CD146 SHM-57 | VCAM-1 STA | VEGFR2 HKDR-1 | CD45 HI30 | PECAM WM59 | CD144 16B1 | CD34 581 | ICAM-1 HCD54 | CD133 293C3 | CD37D6 & CD19 SJ25-C1 | |
B cells# | IgD IA6-2 | CD80 2D10.4 | CD86 B7-2 | CD27 O323 | CD45 HI30 | CXCR4 12G5 | CD23 EBVCS-5 | CD20 2H7 | CD38 HIT2 | CD19 SJ25-C1 | ||
T cells | γδ -TCR B1.1 | CTLA-4 14D3 | CD28 CD28.2 | CD27 O323 | CD45 HI30 | ICOS ISA-3 | CD56 CMSSB | CD62L DREG-56 | CD3 UCHT1 | CD8 OKT-8 | CD4 OKT-4 | |
T cells | CCR7 150503 | CCR5 T21/8 | CXCR3 1C6 | CD27 O323 | CD45 HI30 | CD56 CMSSB | CD62L DREG-56 | CD3 UCHT1 | CD8 OKT-8 | CD4 OKT-4 | ||