Table 1 Primer properties used in qRT-PCR
From: Myostatin and IGF-I signaling in end-stage human heart failure: a qRT-PCR study
Target | Accession number | Forward primer | Reverse primer | Efficiency | Product size (bp) |
|---|---|---|---|---|---|
Mstn | NM_005259.2 | TTCGTCTGGAAACAGCTCCT | CATTTGGGTTTTCCATCCAC | 1.783 | 220 |
ActRIIB | NM_001106.3 | TGACTTTGGCTTGGCTGTTC | ATGTACTCATCCACGGGTCC | 1.834 | 219 |
IGF-I | XM_005268835.1 | ATGCTCTTCAGTTCGTGTGTG | GGGTCTTGGGCATGTCGGTG | 1.758 | 219 |
IGFI-R | NM_000875.3 | GACAACCAGAACTTGCAGCA | GATTCTTCGACGTGGTGGTG | 1.714 | 241 |
HPRT | NM_000194.2 | TGCTCGAGATGTGATGAAGG | TCCCCTGTTGACTGGTCATT | 2.044 | 192 |