Figure 5
From: An autophagosome-based therapeutic vaccine for HBV infection: a preclinical evaluation
Immunized lymphocytes transfer adoptive immunity against HBV. The schematic diagram outlines the experiment protocol (A). HBV+ DRibbles elicited IFN-γ producing cells in 2 × 105 lymphocytes against multiple HBV antigens or peptides (n = 3) (B,C). A total of 1 × 108 lymphocytes were adoptively transferred intravenously into each of the new ‘HBV tolerant’ mouse. At day 7, 14 and 21 after adoptive transfer, serum samples were collected for detection of HBsAg (D), HBV DNA copy number (E). The liver tissues were harvested on day 21 and HBcAg+ hepatocytes were determined as described (n = 4) (F). These experiments were repeated three times with comparable results.