Figure 6

Protein expressions of reactive oxygen species (ROS) and oxidative stress in LV myocardium at 72 h after IR procedure. A) Protein expression of NOX-1 in LV myocardium. p < 0.001, * vs. other groups with different symbols (*, †, ‡). B) Protein expression of NOX-2 in LV myocardium. p < 0.001, * vs. other groups with different symbols (*, †, ‡). C) Protein expression of oxidative index (protein carbonyls) in LV myocardium. p < 0.0001, * vs. other groups with different symbols (*, †, ‡). (Note: right and left lanes shown on the upper panel represent control oxidized molecular protein and standard and protein molecular weight marker, respectively). DNP = 1–3 dinitrophenylhydrazone. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡) indicate significance (at 0.05 level). WT-SC = wide type sham control; WT-IR = wide type + ischemia reperfusion; DDP4^D-SC = dipeptidyl peptidase-IV (DPP4) deficiency sham control; DDP4^D-IR = DDP4^D + ischemia reperfusion; WT-IR-Sita = wide type + ischemia reperfusion + sitagliptin.