Figure 2

Nanobodies against H5N1 were selected by phage display library. (A) The enrichment was detected and H5N1-specific VHHs were enriched about 200-fold after three rounds of panning. (B) Periplasmic extract ELISA for 95 clones for detecting and 26 clones were identified as the positive clones. The ratio higher than 2 was considered as positive. (C) Three kinds of different amino acid sequences of anti-H5N1 VHHs were identified.