Figure 4

The amount of cytokines produced by BsAb-IR+ T-cells is dependent on the presence of the CD28 costimulatory domains within the immune receptor construct. Comparison of cytokine production by T-cells transduced with different BsAb-IR constructs. Pooled supernatant from triplicate co-cultures were measured for proinflammatory cytokine secretion by cytokine bead assay. IFNg, IL-2, TNFa, IL-4 and IL-10 cytokine concentrations (pg/ml) were measured from cultures of BsAb-IR-z, BsAb-IR-28z and control GFP transduced T-cells stimulated with 0.1ug of an immobilized anti-BsAb-IR (MOV18) antibody. Representative data from 4 independent assays are shown.