Figure 4
DC vaccines prepared by Adherence support lymphocyte proliferation and cytokine production. (A) DCs made by the 2 methods were assessed for proliferation by 3H thymidine incorporation at 48, 72, and 96 hours beyond day 8, (the day DCs would have been administered as vaccine). Proliferation responses for Adherence DCs are shown in red and Selected DCs are shown in purple. The results are averages of triplicate wells. * indicates p < 0.05. (B) Supernatants of Adherence and Selected DC cultures were examined for pro-inflammatory cytokines on Day 6 and Day 8. Data for IL-2 is shown for 3 donors. Black line represents the mean. * indicates p < 0.05. NS = not statistically significant.