Figure 6
Post-processing assessment of leukocyte subsets in the TCF. Fractions collected during the CliniMACS CCS process (leukapheresis, original fraction (OF), T-cell fraction (TCF), negative fraction (NF), waste fraction (WF), 48 h, 54 h, and 72 h post-leukapheresis (Stabi48, Stabi54, Stabi72)) were assessed for leukocyte subsets including: CD3+ T cells, CD3+CD56+ NKT cells, CD3−CD56+ NK cells, CD19+ B cells, CD33+ granulocytes and CD14+ monocytes. (A) The compositions of the leukocyte subsets in the Leukapheresis products and the TCFs and (B) the log depletion in cell numbers of leukocyte subsets after CliniMACS CCS enrichment are shown. The results of independent experiments are expressed as the number [x106] of IFN-γ+ T cells ± SD and the fold decrease [log] of cell numbers in leukocyte subsets. Asterisks indicate statistically significant differences between T cells and other leukocyte subsets (*p < 0.05).