Table 1 Taqman primer and probe sequences for real-time PCR
From: Berberine derivatives reduce atherosclerotic plaque size and vulnerability in apoE−/− mice
Gene | Forward primer | Reverse primer | Probe |
|---|---|---|---|
CD68 | CCGAATCCTATACCCAATTC | CTTGGTTTTGTTGGGATTCA | TGGAAGAAAGGTAAAGCT |
α-SMA | CCTCCAGAACGCAAGTACTC | CTTCGTCGTATTCCTGTTTG | CTGGATCGGTGGCTC |
EMMPRIN | CAGAAAACCCACCTGGAAGA | ATAAACCCCTAAGGAATGGA | CAGCCCCTGTTTCT |
MMP9 | TCAAGGATTGCTCAGAGATT | GCAACCACAGTGAGTGAGTT | CCCAGACTCCTCTCT |
NFkB p65 | GGCAGTATTCCTGGCGAGAGA | CCAGGGAGATTCGAACTGTT | ATGGCTACACAGGACC |
ICAM-1 | CCTTAGCAGCTGAACAATCG | TAAGGAGTTGGAACATTTCC | AGGCGGCTCCACC |
VCAM-1 | GACAAGTCCCCATCGTTGAA | ACCTCGCGACGGCATAATTT | CTGGAATGGTCCCATGAT |