Figure 1

Isolation and evaluation of cultured prostate cancer cells. A. LAPC-4 prostate cancer cells spiked into whole blood from healthy donors were sorted onto slide chambers for immunofluorescence (IF) using flow-cytometry techniques based on expression pattern of CD45 and EpCAM. They were evaluated for presence of a nucleus with DAPI and expression of EpCAM and cytokeratin. B. AR-positive LAPC-4 cells and AR-negative DU145 cells spiked into whole blood from healthy donors were sorted onto slide chambers for IF and evaluated for AR staining and localization. C. Ten C4-2 cells spiked into whole blood from a healthy donor and 10 white blood cells (WBCs) were isolated by flow sorting, and extracted DNA underwent whole genome amplification. A portion of the AR gene was subsequently amplified and sequenced using capillary sequencing, and a known T→C mutation was identified.