Figure 5

Influence of GRP78 knockdown and overexpression on eutopic endometrial epithelial cells apoptosis. A: GRP78 expression in separated eutopic endometrial epithelial cells before and after GnRHa treatment was detected through Western blotting. The statistical analysis of the two groups showed obvious differences. *P <0.05 B: The GRP78 knockdown cells exhibited lower GRP78 expression than the control cells. (Left) The knockdown of GRP78 did not affect normal endometrial cell apoptosis, while Leuprolide acetate treatment increased the percentage of apoptosis in the GRP78 knockdown cells compared with the control cells (Right). Ctrl, cells without LA treatment; NC, cells transfected with scrambled control; siRNA-1, cells transfected with siRNA-1 that targeting GRP78; siRNA-2, cells transfected with siRNA-2 that targeting GRP78. *P <0.05 vs NC within the Ctrl goup, #P <0.05 vs NC within the LA goup. C: Comparison of the GRP78 protein levels in GRP78 overexpression and control groups. (Left); GRP78 overexpression did not affect normal endometrial cell apoptosis, while GRP78 overexpression protected the cells from Leuprolide acetate-induced apoptosis (Right). Ctrl, cells without LA treatment; NC, cells transfected with pCMV-3X Flag; GRP78, cells transfected with pCMV-3X Flag-GRP78. *P <0.05 vs NC within the Ctrl goup, #P <0.05 vs NC within the LA goup.