Table 1 Sequences for target gene primers for qRT-PCR
Gene | Primer sequence 5’-3’ | Tm (°C) |
|---|---|---|
RNU6B | F: CTCGCTTCGGCAGCACA | 59.42 |
R: AACGCTTCACGAATTTGCGT | 55.75 | |
miR-200c | F: TAATACTGCCGGGTAATGATGG | 58.21 |
R: TCGTATCCAGTGCAGGGTC | 59.72 | |
GAPDH | F: TGCACCACCAACTGCTTAG | 60.07 |
R: AGTAGAGGCAGGGATGATGTTC | 59.72 | |
Bmi1 | F: TGGATCGGAAAGTAAACAAAGAC | 56.60 |
R: TGCATCACAGTCATTGCTGCT | 58.01 | |
E2F3 | F: TGCCTGACTCAATAGAGAGCCTAC | 61.97 |
R: TCCCATTGTGGTCTTGGTTGT | 58.01 | |
E-cadherin | F: GAAAGCGGCTGATACTGACC | 59.85 |
R: CGTACATGTCAGCCGCTTC | 59.72 | |
Vimentin | F: GGGAGAAATTGCAGGAGGAG | 59.85 |
R: AGGTCAAGACGTGCCAGAGAC | 61.92 |