Expression and prognostic value of TCTN1 in a Chinese glioblastoma (GBM) cohort. (A) TCTN1 protein expression was analysed by immunohistochemistry staining in human GBM specimens and normal brain samples, and representative images show high nuclear expression of TCTN1 in GBMs. Magnification: ×50, left; ×200, right. Scale bars: 100 μm, left; 25 μm, right. (B) TCTN1 mRNA expression was analysed by real time RT-PCR assay in human GBM samples, and GAPDH was used as an internal control. P value was determined by Student's t test. (C-D) Kaplan-Meier plots were estimated according to different TCTN1 immunoreactivity level for overall survival (C) and progression-free survival (D) of GBM patients. P values were obtained from log-rank test, and hazard ratio (HR) and 95% confidence interval (CI) were calculated by univariate Cox regression model.