Table 2 Primers of DR3, PDCD5, p53 and FasL for real-time PCR
Target gene | Locus | Sense sequence(5'-3') | Antisense sequence(5'-3') | Product size(bp) |
|---|---|---|---|---|
DR3 | NM_001137644.1 | gctacgaacctacaacataccg | acagtcccaagaaggaacgag | 181 |
PDCD5 | NM_001106247.1 | acgaaagcagtggagaactacc | ttcttttctgtctgttggctga | 119 |
p53 | NM_030989.3 | gcccatccttaccatcatcac | cacaaacacgaacctcaaagc | 80 |
FasL | NM_012908.1 | ggtgctaatggaggagaagaag | aaatggtcagcaacggtaagat | 105 |
DNMT3a | NM_001003958.1 | gtgcttaccaatacgatgacga | atccacacactccacacaaaag | 122 |
DNMT3b | NM_001003959.1 | ggtaggagatggagatggtgaa | atactgttgctgtttcgggttc | 138 |
DNMT1 | NM_053354.3 | gtgtggtgtctgtgaggtctgt | gtttcttcttcttcccttggtg | 221 |
β-actin | NM_001009180.2 | tgacaggatgcagaaggaga | tagagccaccaatccacaca | 106 |