Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Table 1 Preliminary experiments: gradient centrifugation parameters do not affect impurities' removal nor cell yield (n = 5)

Gradient medium	Centrifugation parameters	% PLT removal	% GRA removal	% MNC yield
Standard Ficoll-Paque (1.077 g/L)	719 × g, 20°C, 20'	93.73 × 1.63	96.02 × 3.05	24.15 × 22.90
Standard Ficoll-Paque (1.077 g/L)	400 × g, 20°C, 30'	92.05 × 1.36	93.41 × 1.64	27.41 × 15.98

Five sternal bone marrow samples were processed in parallel with the same gradient medium but using different centrifugation parameters, as indicated. Data are reported as mean ± standard deviation. No statistically significant differences were detected (p > 0.05, paired T-test).
PLT: Platelets; GRA: Granulocytes; MNC: Mononuclear cells (lymphocytes +μmonocytes).

ISSN: 1479-5876