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Table 1 Preliminary experiments: gradient centrifugation parameters do not affect impurities' removal nor cell yield (n = 5)

From: Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Gradient medium Centrifugation parameters % PLT removal % GRA removal % MNC yield
Standard Ficoll-Paque (1.077 g/L) 719 × g, 20°C, 20' 93.73 × 1.63 96.02 × 3.05 24.15 × 22.90
400 × g, 20°C, 30' 92.05 × 1.36 93.41 × 1.64 27.41 × 15.98
  1. Five sternal bone marrow samples were processed in parallel with the same gradient medium but using different centrifugation parameters, as indicated. Data are reported as mean ± standard deviation. No statistically significant differences were detected (p > 0.05, paired T-test).
  2. PLT: Platelets; GRA: Granulocytes; MNC: Mononuclear cells (lymphocytes +μmonocytes).
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