Curcumin suppresses MMP-9, MMP13 and EMMPRIN expression in PMA induced macrophages. A. Effect of curcumin on cell viability. PMA-induced macrophages were treated with indicated concentrations of curcumin (5–100 μM) for 48 h, and cell viability was assessed using the CCK-8 assay. Cells incubated in a medium without curcumin were defined as a control and were considered 100% viable. B-D. Curcumin suppresses EMMPRIN, MMP-9 and MMP13 expression in PMA-induced macrophages. The mRNA level and protein level were measured by Real-time PCR and Western blot, respectively. *P < 0.05 vs PMA group, **P < 0.05 vs CTL group. CTL indicates that cells incubated in a medium without curcumin. 6.25 μM, 25 μM, 50 μM indicates that THP-1 cells were pretreated with indicated concentrations of curcumin before stimulating with 100 nM PMA for another 48 h. Cur: curcumin.