Figure 3

EGFR, HSP and costimulatory molecules expression on HNSCC cells. (A) HNSCC tumor cells were incubated with erlotinib (1 μM) for 48 h and the expression of EGFR, pEGFR, HSP70, and HSP90 was examined by western blot. (B) CD80, CD86, and PD-L1 expression on HNSCC cell lines HSC-4 and Sa-3 was examined by flow cytometry. HNSCC cells were treated with IFN-γ (50 U/ml) alone (red line) or IFN-γ and erlotinib (1 μM; blue line) for 48 h.