Figure 2

Flow cytometric characterization of B cells, T cells, NK cells, NKT cells, and neutrophils in a representative sample of psoriatic adipose tissue. After exclusion of debris, doublets, and non-viable cells, CD16-CD19+ B cells, CD3+CD56- T cells, CD3-CD16-CD56^Hi NK cells, CD3+CD56+ NKT cells, and total CD16+ cells were identified. CD16+ cells were divided into neutrophils (CD16+SSC^Hi) and NK cells (CD16+CD56^LoSSC^Lo) based on SSC properties. CD3+ T cells were further gated into naïve and effector and/or memory (eff/mem) αβ T cell subsets, CD4+CD8- T cells, CD4-CD8+ T cells, γδ T cells, and T regulatory (FoxP3+) cells. All cell populations are presented as percentages of viable cells except for granzyme B, IFN-γ, and TNF-α expressing cells, which are reported as percentages of the parent population. Positive gating for each fluorochrome parameter was established using FMO controls.