Bioenergetic modulation with dichloroacetate reduces the growth of melanoma cells and potentiates their response to BRAFV600E inhibition

Table 1 DCA IC ₅₀ values, BRAF/NRAS status and PGC1α expression

Cell line	DCA IC₅₀(mM)⁴	BRAF/ NRASStatus¹	PGC1α expression^2,4
ED-070	8.9 ± 0.6***	NRAS^Q61L	1.0 ± 0.2
ED-007	12.2 ± 2.2***	WT³	0.6 ± 0.0
ED-071	12.3 ± 3.6***	BRAF^V600E	0.0 ± 0.0
ED-034	12.7 ± 1.7***	BRAF^L597S	1.0 ± 0.2
ED-013	14.4 ± 2.0***	BRAF^V600E	1.9 ± 0.3
ED-027	17.7 ± 2.1***	BRAF^V600E	0.5 ± 0.1
SK-MEL-28	20.0 ± 4.5***	BRAF^V600E	0.0 ± 0.0
ED-179	20.6 ± 1.8***	NRAS^Q61R	0.3 ± 0.0
ED-024	21.9 ± 1.6***	NRAS^Q61L	0.0 ± 0.0
ED-140	23.9 ± 2.0***	WT	0.4 ± 0.1
ED-050	24.1 ± 3.1***	WT	2.7 ± 0.5
ED-029	29.7 ± 5.1***	BRAF^V600K	1.3 ± 0.2
ED-196	35.8 ± 3.2***	BRAF^V600E	1.5 ± 0.5
ED-117	37.6 ± 2.2***	BRAF^V600E	1.2 ± 0.1
HEMn-LP	69.1 ± 6.4	WT	1
ED-013-R1	12.6 ± 3.0***	BRAF^V600E
ED-013-R2	13.6 ± 2.4***	BRAF^V600E
ED-071-R1	12.2 ± 0.9***	BRAF^V600E
ED-071-R2	13.8 ± 3.7***	BRAF^V600E
SK-MEL-28-R1	23.1 ± 4.6***	BRAF^V600E
SK-MEL-28-R2	26.2 ± 8.0**	BRAF^V600E

**p < 0.01; ***p < 0.001 when compared with HEMn-LP.
¹BRAF/NRAS status are in accordance with published results [25].
²Relative to PGC1α expression in normal melanocytes.
³wild type.
⁴DCA IC₅₀ values and PGC1α expression represent the means of three independent measurements ± standard deviation.

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