Figure 1

Metabolic characterization of melanoma cell lines and primary melanocytes. A, Metabolic profiles of two melanoma cell lines (ED-013 and ED-179) and human epidermal melanocytes (HEMn-LP), based on Seahorse XF96 measurements. Depicted are the ECAR (left panel) and the OCR (right panel) measurements during successive addition of oligomycin (1 μM), FCCP (1 μM), rotenone/antimycin (1 μM/1 μM) and 2-DG (100 mM). Data constitute 6 parallel measurements, and are representative of three independent experiments. B, Basal and maximal glycolytic ECAR values for melanoma cell lines and human primary melanocytes (HEMn-LP). The ECAR measured after addition of 2-DG (non-glycolytic ECAR) was subtracted from all values. The dashed line indicates the basal ECAR of HEMn-LP. C, Ratios between basal mitochondrial OCR and basal glycolytic ECAR. D, ATP coupling representing the fraction of the basal OCR used for ATP production (fraction inhibited by oligomycin). E, The respiratory control ratio, denoting the ratio between the maximal mitochondrial OCR and the proton leak (OCR after addition of oligomycin). (B – E), Values are the means of three independent measurements ± standard deviation. Students t-test was used to determine differences between HEMn-LP and melanoma cell lines (*p < 0.05; **p < 0.01; ***p < 0.001).