Culture and identification of UC-MSCs. (A) UC-MSCs exhibiteda spindle- and fibroblast-like shape. (B-D) Multipotential differentiation of UC-MSCs. UC-MSC differentiation into adipocytes, osteoblasts and chondroblasts, as shown by Oil Red O (B), alkaline phosphatase (C), and alcian blue (D) staining, respectively, of in vitro differentiation cultures. (E-L) Flow cytometry analysis of surface markers inUC-MSCs. Most of the cultured cells were positive for expression of CD90 (E), CD73 (F) and CD105 (G) but were negative for expression of CD45 (H), CD34 (I), CD14 (J), CD19 (K) and HLA-DR (L). The scale bar is 10 μm in A, 100 μm in B and C, and 50 μm in D.