miR-10b promotes HCC cell migration and invasion. (a) In MHCC-97L cells transfected with pcDNA3.1-miR-10b and control vector and in MHCC-97H cells transfected with anti-miR-10b, miR-10b expression was measured by real-time quantitative RT-PCR. (b) Proliferation of MHCC-97H and MHCC-97L cells transfected with miR-10b inhibitor or miR-22 overexpression vector was measured in the indicated time periods using the MTT assay. (c) Wound healing assay of MHCC-97H and MHCC-97L cells transfected with miR-10b inhibitor or miR-22 overexpression vector. (d) Migration and invasion potential in both cell lines treated as above were measured by Transwell assays. The results were the means of three independent experiments ± S.D. * P < 0.05, Student’s t test was used to analyze the significant differences.