The proportion of NKT cells in peripheral blood. Lymphocytes were gated from all RBC-free single cell suspensions by FSC and SSC, and CD3+NK1.1+ cells were selected in all lymphocytes. Isotype group was performed as control (A). After Rapamycin treatment, the proportion of NKT cells (CD3+NK1.1+) was significantly increased compared with IR group and Sham group (B). Data are expressed as mean ± SD (n = 5).