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A rare polymorphism in Toll Like Receptor 2 is associated with systemic sclerosis phenotype and increases production of inflammatory mediators

Background

Toll like receptors play an important role in fine-tuning innate immune responses, but genetic variations in TLR genes have been shown previously to augment immune responses and susceptibility to autoimmune disease.

Aim

To investigate whether polymorphisms in toll like receptor (TLR) genes, previously reported to be associated with immune mediated diseases are implicated in systemic sclerosis (SSc).

Methods

We genotyped 14 polymorphisms in the TLR 2, 4, 7, 8 and 9 genes in a discovery cohort comprising 452 SSc patients and 537 controls and a replication cohort consisting of 1170 SSc patients and 925 controls. Furthermore we analyzed 15 year follow-up data from 964 patients to assess the potential association of TLR variants with the development of disease complications. Next to this, we analyzed the functional impact of the associated polymorphism on monocyte derived and myeloid dendritic cells.

Results

Exploiting the discovery cohort, we observed that a rare functional polymorphism in TLR2 (Pro631His), was associated with anti-topoisomerase positivity (p=0.003 OR 2.24 95%CI:1.24-4.04). This observation was validated in the replication cohort (p=0.0001 OR 2.73 95%CI:1.85-4.04). In addition, the replication cohort also revealed an association between the TLR2 variant with the diffuse subform of the disease and the development of pulmonary arterial hypertension, respectively (p=0.02, Log-Rank p=0.003, Cox proportional hazards ratio: 5.61 ((95%CI 1.53-20.58)). Functional analysis revealed that monocyte derived dendritic cells carrying the Pro63His variant produce more inflammatory mediators (TNFalpha and IL-6) upon TLR2 mediated stimulation (both p<0.0001).

Conclusion

The rare TLR2Pro631His variant is robustly associated with anti-topoisomerase positivity, diffuse SSc and the development of PAH. Besides, this variant influences TLR2 mediated cell responses. Further research is necessary to reveal the precise role of TLR2 in the disease pathogenesis of SSc.

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Correspondence to Jasper CA Broen.

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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Broen, J.C., Bossini-Castillo, L., Bon, L.v. et al. A rare polymorphism in Toll Like Receptor 2 is associated with systemic sclerosis phenotype and increases production of inflammatory mediators. J Transl Med 9, O4 (2011). https://doi.org/10.1186/1479-5876-9-S2-O4

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Keywords

  • Dendritic Cell
  • Toll
  • Toll Like Receptor
  • Pulmonary Arterial Hypertension
  • Systemic Sclerosis