Figure 5

F11R protein expression in endothelial cells treated with TNFα and INFβ. (a). Immunoblotting: HAEC or HUVEC cells were treated with TNFα (100 u/mL), IFNγ (200 u/mL) or TNFα (100 u/mL) and IFNγ (200 u/mL) for 24 hrs. Collected media and cell lysates were examined for the presence of the F11R protein by SDS-PAGE (10%) followed by immunoblotting utilizing antibodies against F11R and tubulin (protein loading control, 50 kDa). (b). Quantitation of immunoblots - cell lysates. Enhanced expression of the F11R protein in cytokine-treated human aortic endothelial cells (HAEC) and umbilical vein endothelial cells (HUVEC). Quantitation of the F11R protein in cell lysates of the TNFα and/or IFNγ-treated HUVEC and HAEC, as detailed in the legend of Figure 5a. Immunoblots derived, following SDS-PAGE, were immunostained utilizing an F11R antibody. The level of the immunostained F11R protein band, of 37 kDa, was normalized to tubulin, the loading protein control, of 50 kDa. Values represent the mean ± SEM. * P < 0.05. (c). Quantitation of immunoblots - cell media. Quantitation of the F11R protein detected in the cell culture media of TNFα and/or IFNγ-treated HUVEC and HAEC (as detailed in the legend of Figure 5a), normalized to input volume. Values represent the mean ± SEM. * P < 0.05.