Figure 1

Expression of F11R mRNA in human aortic endothelial cells (HAEC) and umbilical vein endothelial cells (HUVEC) exposed to proinflammatory cytokines TNFα and/or IFNγ: time course. Real-time PCR was performed in cultured HAEC (top panels) treated for 0, 3, 6, 12, and 24 hrs with TNFα (100 u/mL) and/or IFNγ (200 u/mL), and in cultured HUVEC (bottom panels) treated for 0,4,8,12, and 24 hrs with TNFα (100 u/mL) and/or IFNγ (200 u/mL). Real-time PCR was performed three times in triplicate for each time point. Values represent the mean ± SEM. *P < 0.05 indicates the level of significance determined at a specific interval of time of cytokine- treatment of ECs in comparison to the zero time points.