Figure 3

MTSS1 expression in oesophageal cancer cell lines and over-expression and knockdown of MTSS1. (A) RT-PCR analysis of MTSS1 mRNA expression within a panel of oesophageal cancer cell lines. MTSS1 was not expressed in KYSE150 oesophageal squamous cell carcinoma cells, but expressed in other three types of oesophageal cancer cell lines. (B) Verification of over-expression and knockdown of MTSS1 in the oesophageal cancer cell lines using RT-PCR. MTSS1 was over-expressed in the KYSE150-MTSS1-Exp cells; whereas MTSS1 expression levels were reduced in the KYSE510-MTSS1-Rib cells. (C) Western blotting confirmation of MTSS1 protein level.