Changes in CD3+ T cell frequency and distribution following LA treatment of selected liver metastases. Groups of mice (n >5) had two tumors LA treated and tissues were collected at various time points post ablation from areas indicated in the diagram of panel a. Control mice had sham LA performed (LA probe was not activated). Panels b-d are CD3+ stained tissues from control animals; b liver parenchyma, c tumor host interface and d an enlarged section of c as shown in the rectangle. Panel e depicts a section of liver parenchyma from an LA treated animal immediately following ablation. Panels f-l are CD3+ stained tumor sections from LA treated animals collected at day 2 post treatment. Panels f and g depict CD3+ staining of a vascular lake in a distant tumor; g is an enlarged section of panel f as shown in the rectangle. Panel h depicts a section of distant tumor host interface Panels i and j depict sections of the ablated tumor injury front. Panels k and l depict sections of the ablated tumor host interface. Panels c, f and i original magnification 50x, all other panels original magnification 200x. L, liver; T, tumor; N, necrotic tissue within the injury front.