Figure 4
Western blot analysis of different BRAFV600E mutant cell lines. Cells were exposed for 24 hours to 5 μM vemurafenib, 10 mM metformin, or the combination. Phosphorylation was analyzed with antibodies against specific phosphor-proteins compared to their total proteins. Synergistic characteristics of the cell lines are indicated as + (slight synergy), ++ (significant synergy), - (slight antagonism), and - - (significant antagonism).