Figure 3From: Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutationsEffects of single agent and combination therapy on apoptosis. To test the cytotoxic effects of the different treatments, melanoma cells were treated for 72 hours with 5 μM vemurafenib, 10 mM metformin, or the combination. Cells were stained with Annexin V and PI and were analyzed by flow cytometry. a) Annexin/PI staining of M263 as a representative example. Upper left corner: DMSO, upper right corner: vemurafenib, lower left corner: metformin, lower right corner: Combination of vemurafenib and metformin. b) Early and late apoptotic cells after 72-hour treatment with the indicated agents. Percentages are shown in relation to the control, DMSO treated cells.Back to article page