Figure 1

Transduction of purified PEP-1-SOD1 or PEP-1-CAT into H9c2 cells. A: purification of PEP-1-SOD1-His and PEP-1-CAT-His fusion proteins. Purified fusion proteins were analyzed by western blot with rabbit anti-polyhistidine antibody. B: H9c2 cells were treated with 2 μM purified His-tagged PEP-1-SOD1, wild type SOD1, PEP-1-CAT, or wild type CAT proteins for 6 h. Cells were incubated with rabbit-anti-polyhistidine and mouse-anti Troponin T (cardiomyocyte marker) antibodies (cTnT), and then visualized with fluorescent microscopy. Red fluorescent signals represent TRITC-labeled His-tag of SOD1 or CAT, Green fluorescent signals represent FITC-labeled Troponin T; Blue fluorescent signals represent DAPI-labeled nuclei.