Table 1 Enrichment performance of the three different methods after spiking 100 SW620 cells in 5 mL peripheral blood (all assays were repeated 3 times).
Method | Total number of leukocytes | Recovery | Purity | ||
|---|---|---|---|---|---|
| Â | Before enrichment | After enrichment | Average (%) | Range (%) | Average (%) |
CD45 depletion | 3 × 107 | 6.0 × 103 | 58 | 50-66 | 0.97% |
Positive enrichment | 3 × 107 | 2.0 × 103 | 25 | 24-26 | 1.25% |
CD45 depletion + positive enrichment | 3 × 107 | 1.5 × 103 | 22.5 | 20-25 | 1.50% |