Figure 1

Signature of IFNα on human PBMC 24 hours after the cytokine administration. Treatment schedules for the clinical trials on melanoma patients (A) and healthy donors (C) examined in this study (see Methods and Additional data file 1). (B) Clusterogram showing the supervised hierarchical clustering of 6 PBMC samples collected before (T0, T42, blue bar) or after (T2, T44, red bar) the first and the fourth administration of IFNα plus melanoma peptides. The analysis was restricted to the 1093 most informative transcripts among the 17,000 of the complete dataset (80% gene presence across all experiments and at least 3-fold ratio change). (D) Clusterogram showing the supervised hierarchical clustering of all samples collected before (T0, T1m, blue bar) or after (T0+24, T1m+24, red bar) the first and the second cycle of administration of HBV vaccine in combination with placebo (black bar, group A), 1 (green bar, Group B) or 3 MU of IFNα (orange bar, Group C). The analysis was restricted 1712 most informative genes (see above). The enlargements show the nodes of genes specifically up-regulated after each IFNα plus vaccine administration.